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1.
Food Res Int ; 178: 113901, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38309860

RESUMO

The Maillard reaction occurs during the frying of batter-coated meat products, resulting in the production of advanced glycosylation products that are harmful to human health. This study investigated the effects of frying temperature (140, 150, 160, 170 and 180 ℃) and time (80, 100, 120, 140 and 160 s) on the quality, advanced glycation end product (AGE) level and the relationship between these parameters in batter-coated meat products were investigated. The results showed that with an increase in frying temperature and time, the moisture content of the batter-coated meat products gradually decreased, the thiobarbituric Acid Reactive Substance (TBARS) values and oil content increased to 0.37 and 21.7 %, respectively, and then decreased, and CML and CEL content increased to 7.30 and 4.86 mg/g, respectively. Correlation analysis showed that the moisture content and absorbance at 420 nm, as well as TBARS values, were highly correlated with the oil content in batter-coated meat products. Additionally, the absorbance at 420 nm and TBARS levels were significantly correlated with AGE levels. Moreover, the AGE content in batter-coated meat products was less variable at lower frying temperatures or shorter frying times, and the influence of temperature on AGE formation was greater than that of time. Overall, these findings may help to better control the cooking conditions of batter-coated meat products based on AGE profiles.


Assuntos
Reação de Maillard , Produtos da Carne , Humanos , Produtos Finais de Glicação Avançada/análise , Produtos da Carne/análise , Substâncias Reativas com Ácido Tiobarbitúrico , Lipídeos
2.
Food Chem X ; 21: 101069, 2024 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-38292686

RESUMO

Foodborne spores are ubiquitous with extremely strong resistance, and pose a serious threat to food safety and human health. Therefore, rapid, sensitive, and selective detection of spores are crucial. In this study, a fluorescent probe was developed based on lanthanide ion (Eu3+)-labeled nano-silver-modified graphene oxide (GO-AgNPs-Eu3+) for the detection of 2,6-dipicolinic acid (DPA), a biomarker unique to spores, to allow quantitative spores detection. The GO-AgNPs-Eu3+ nano-fluorescent probe was loaded onto a polyvinylidene fluoride microfiltration membrane, and a smartphone-assisted portable GO-AgNPs-Eu3+ nanoparticles-based paper visual sensor was designed for rapid on-site quantitative and real-time online detection of spores. The results indicated that the developed probe achieved equilibrium binding with DPA within 5 min, and enhanced fluorescence emission through antenna effect. The fluorescence detection presented a good linear relationship in the DPA concentration range of 0-45 µM, with a DPA detection limit of 4.62 nM and spore detection limit of 104 cfu/mL. The developed sensor showed a change in fluorescence from blue to red with increasing DPA concentration, and this color change was quantitatively detected through smartphone RGB variations, with a detection limit of 13.1 µM for DPA and 6.3 cfu/mL for Bacillus subtilis spores. Subsequently, the sensitivity and selectivity of the developed sensor were verified using actual milk and water samples spiked with B. subtilis spores. The results of this study provided objective technological support for rapid detection of spores, which is important for reducing the occurrence of foodborne diseases and improving food safety.

3.
Int J Biol Macromol ; 261(Pt 2): 129574, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38246464

RESUMO

In order to explore the influence of wood types on formation of polycyclic aromatic hydrocarbons (PAHs) in traditional smoked and grilled meat products, the effect of lignin in woods on formation of PAHs was investigated in meat model systems. The results showed that PAHs formation was much dependent on the heating conditions. The addition of lignin led to significantly increased PAHs, which being connected with lignin structure. In comparison, the formation of PAHs was more facilitated by lignin with G structure than that with G/S structure. However, further study of adding lignin precursors demonstrated that lignin precursors with S structure were more favorable to the formation of PAHs than those with G structure. It was proposed that the relative content and activity of G/S structure of lignin in wood played a significant role in the formation of PAHs, which might provide theoretical reference for inhibition of PAHs fundamentally.


Assuntos
Produtos da Carne , Hidrocarbonetos Policíclicos Aromáticos , Produtos da Carne/análise , Lignina , Culinária/métodos , Hidrocarbonetos Policíclicos Aromáticos/análise , Fumaça , Carne/análise
4.
Anal Methods ; 16(2): 284-292, 2024 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-38113049

RESUMO

A study on the inactivation and germination mechanism of spores is very important in the application of spores, as such high-purity spores are the basis of related research. However, spores and vegetative cells of bacteria often coexist, and it is difficult to separate them. In this study, a magnetic flow device for the purification of spores in the culture medium system was developed based on a "stepped" structure with a magnetic force that could absorb vegetative cells with magnetic nanoparticles. The operation process was as follows: first, vancomycin functionalized nanoparticles were used to prepare Van-Fe3O4 NPs, which were then combined with vegetative cells to form a magnetic conjugate. Subsequently, the magnetic conjugate (vegetative cells) flowed through the "stepped" magnetic flow device and was adsorbed. Meanwhile, the spores moved through the channel and were collected. The achieved purity of the collected spores was more than 95%. Further, the number of the obtained spores was quickly quantified using Raman spectroscopy. The entire purification and quantitative process can be completed within 30 min and the limit of detection was 5 CFU mL-1. This study showed outstanding spore purification ability and provided a new method for purification and rapid quantitative detection of spores.


Assuntos
Esporos Bacterianos , Esporos , Esporos Bacterianos/fisiologia , Bactérias , Meios de Cultura , Fenômenos Magnéticos
5.
Foods ; 12(24)2023 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-38137319

RESUMO

The emulsifying capacity of bovine bone protein extracted using high-pressure hot water (HBBP) has been determined to be good. Nevertheless, given that HBBP is a blend of peptides with a broad range of molecular weights, the distinction in emulsifying capacity between polypeptide components with high and low molecular weights is unclear. Therefore, in this study, HBBP was separated into three molecular weight components of 10-30 kDa (HBBP 1), 5-10 kDa (HBBP 2), and <5 kDa (HBBP 3) via ultrafiltration, and the differences in their structures and emulsifying properties were investigated. The polypeptide with the highest molecular weight displayed the lowest endogenous fluorescence intensity, the least solubility in an aqueous solution, and the highest surface hydrophobicity index. Analysis using laser confocal Raman spectroscopy showed that with an increase in polypeptide molecular weight, the α-helix and ß-sheet contents in the secondary structure of the polypeptide molecule increased significantly. Particle size, rheological characteristics, and laser confocal microscopy were used to characterize the emulsion made from peptides of various molecular weights. High-molecular-weight peptides were able to provide a more robust spatial repulsion and thicker interfacial coating in the emulsion, which would make the emulsion more stable. The above results showed that the high-molecular-weight polypeptide in HBBP effectively improved the emulsion stability when forming an emulsion. This study increased the rate at which bovine bone was utilized and provided a theoretical foundation for the use of bovine bone protein as an emulsifier in the food sector.

6.
Mikrochim Acta ; 190(12): 472, 2023 11 21.
Artigo em Inglês | MEDLINE | ID: mdl-37987841

RESUMO

A new surface-enhanced Raman spectroscopy (SERS) biosensor of Graphene@Ag-MLF composite structure has been fabricated by loading AgNPs on graphene films. The response of the biosensor is  based on plasmonic sensing. The results showed that the enhancement factor of three different spores reached 107 based on the Graphene@Ag-MLF substrate. In addition, the SERS performance was stable, with good reproducibility (RSD<3%). Multivariate statistical analysis and chemometrics were used to distinguish different spores. The accumulated variance contribution rate was up to 96.35% for the top three PCs, while HCA results revealed that the spectra were differentiated completely. Based on optimal principal components, chemometrics of KNN and LS-SVM were applied to construct a model for rapid qualitative identification of different spores, of which the prediction set and training set of LS-SVM achieved 100%. Finally, based on the Graphene@Ag-MLF substrate, the LOD of three different spores was lower than 102 CFU/mL. Hence, this novel Graphene@Ag-MLF SERS substrate sensor was rapid, sensitive, and stable in detecting spores, providing strong technical support for the application of SERS technology in food safety.


Assuntos
Grafite , Esporos Bacterianos , Reprodutibilidade dos Testes , Análise Espectral Raman , Quimiometria
7.
Educ Technol Res Dev ; : 1-18, 2023 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-37359483

RESUMO

The outbreak of the coronavirus disease 2019 (COVID-19) promoted online teaching on an unprecedented scale, raising researchers' attention to the importance of faculty's acceptance of this urgent teaching shift. This study aimed to explore the influence of organizational factors on faculty's acceptance of online teaching in terms of behavioral intention and perceived usefulness. A multilevel structural equation model was employed to analyze data on 209,058 faculty in 858 higher education institutions based on a nationwide survey conducted in mainland China. The results showed that three key organizational factors, namely strategic planning, leadership, and teaching quality monitoring, impacted faculty's acceptance of online teaching, although in different ways. Strategic planning had a direct impact on perceived usefulness, while leadership had a direct impact on behavioral intentions, and teaching quality monitoring had a direct impact on both perceived usefulness and behavioral intentions. In addition, an indirect effect was found between strategic planning and faculty's behavioral intentions through the mediation of the perceived usefulness of online teaching. The findings of this study have practical implications for college administrators and policymakers, which should effectively implement and promote online teaching and learning, and consider key organizational factors to increase faculty acceptance.

8.
Foods ; 12(9)2023 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-37174372

RESUMO

Bacteriostats, as chemical substances that inhibit bacterial growth, are widely used in the sterilization process; however, their effects on spindle spores are unclear. In this study, the effects of bacteriostats, including nine commonly used food additives and four detergents, on the growth of Clostridium perfringens spores were investigated. The results showed that 0.07‱ ethylenediaminetetraacetate had a good inhibitory effect on C. perfringens spore growth, and the spore turbidity decreased by 4.8% after incubation for 60 min. Furthermore, 0.3‱ tea polyphenols, 0.8‱ D-isoascorbic acid, and 0.75‱ potassium sorbate promoted leakage of contents during spore germination. Among the four detergents, 5‱ glutaraldehyde solution presented the best inhibitory effect on the growth of C. perfringens spores, and the spore turbidity decreased by 5.6% after incubation for 60 min. Further analysis of the inactivation mechanism of spores by the bacteriostats was performed by comparing the leakage of UV-absorbing substances during germination. The results revealed that bacteriostats could not directly kill the spores, but could inactivate them by inhibiting germination or damaging the spore structure during germination, thus preventing the formation of bacterial vegetative bodies. These findings provide important information and reference for the mechanism underlying the effects of different bacteriostatic agents on spore growth.

9.
Int J Food Microbiol ; 396: 110200, 2023 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-37119648

RESUMO

Clostridium perfringens can form metabolically dormant spores that can survive in meat preservation processes and cause food spoilage and human disease upon germination and outgrowth. The characteristics of spores in food products are closely related to the sporulation environment. To control or inactivate C. perfringens spores in food industry, the effects of sporulation conditions on the spores characteristics should be examined. This study aimed to investigate the effects of temperature (T), pH, and water activity (aw) on the growth, germination, and wet-heat resistance of C. perfringens C1 spores isolated from food product. The results showed that C. perfringens C1 spores produced at T = 37 °C, pH = 8, and aw = 0.997 had the highest sporulation rate and germination efficiency and lowest wet-heat resistance. A further increase in pH and sporulation temperature reduced the spore counts and germination efficiency, but enhanced spores' wet-heat resistance. By using air-drying method and Raman spectroscopy analysis, the water content, composition, and levels of calcium dipicolinic acid, proteins, and nucleic acids in spores produced under different sporulation conditions were determined. The results obtained revealed that sporulation conditions should be carefully considered during food production and processing, thus providing a novel insight into prevention and control of spores in food industry.


Assuntos
Clostridium perfringens , Esporos Bacterianos , Humanos , Contagem de Colônia Microbiana , Temperatura , Temperatura Alta , Água/análise
10.
Food Res Int ; 160: 111426, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36076367

RESUMO

In this study, we developed a novel and sensitive AgNPs self-assembled solid-phase (AgNPs-SASP) SERS substrate platform using seed-mediated and liquid interface self-assembly methods and applied the platform for the detection of three bacterial spores. Multivariate statistical analysis (HCA and LDA) were employed for identification and categorization of the obtained data. The results illustrated that AgNPs-SASP exhibited high reproducibility and Raman enhancement effect (1.43 × 104). The Raman shift bands of the three bacterial spores ranged from 400 to 1800 cm-1 demonstrating the difference in reigon and intensity. And the intensities of their Ca2+-DPA Raman bands are significantly different. HCA results revealed that the spectra for the three bacterial spores were statistically different while LDA completely differentiated the spectra for the three bacterial spores with 100 % sensitivity and specificity. Overall, the novel SERS platform based on AgNPs-SASP provides an effective tool for food safety risk control and detection.


Assuntos
Análise Espectral Raman , Esporos Bacterianos , Análise Multivariada , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise Espectral Raman/métodos
11.
Front Psychol ; 13: 916497, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35874385

RESUMO

The use of information and communication technologies (ICT) is increasingly becoming prevalent among students, both at home and school. While inconsistent results were found for student ICT use and reading literacy, this study attempted to explain these ambiguous links with the moderation of ICT use intensity and mediation of metacognition. Three moderated mediation models for each type of ICT use (at home for entertainment activities and for schoolwork, as well as at school) were analyzed using a Hong Kong sample taken from the Programme for International Student Assessment (PISA) 2018 data pertaining to 5180 15-year-old students from 152 schools. A dynamic effect pattern was found for the links of all ICT use types and reading literacy with the increasing intensity of ICT use, which begins with a positive effect followed by a decrease to less positive, then turns to fluctuating negative and finally ends up with a stable negative effect. But the dominant effect varies across ICT use intensity, which result in different overall effects of three ICT use types. In addition, all three aspects of metacognition showed a profound negative mediation on links of intensive and excessive ICT use with reading literacy, and a less positive mediation for limited ICT use. The metacognition of assessing credibility showed a more important role than summarizing, which was followed by understanding and remembering. In light of the findings, the study recommended that more metacognitive scaffolds should be developed for students with intensive or excessive ICT use, so as to alleviate the side effects of ICT use on their reading literacy.

12.
Biosens Bioelectron ; 213: 114458, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-35714495

RESUMO

In this study, a power-free biosensor was presented to detect Salmonella typhimurium on a microfluidic chip using a slide multivalve for channel selection and a disposable syringe for fluidic transfer. First, bacterial sample with immunomagnetic nanoparticles (IMNPs) and glucose oxidase (GOx) modified immune polystyrene nanoparticles (IPNPs), washing buffer, glucose, and peroxide test strip (PTS) were preloaded in their respective chambers at the periphery of chip. After the slide multivalve was selected to connect sample chamber with common separation chamber, which was connected with a syringe, the mixture of Salmonella, IMNPs and IPNPs was back and forth moved through 3D Tesla-structure micromixer using the syringe, resulting in the formation of IMNP-Salmonella-IPNP complexes, which were captured in the separation chamber using a magnet. Then, two washing chambers were selectively connected respectively to remove sample background and excessive IPNPs, and glucose chamber was connected, allowing the GOx to catalyze glucose to produce hydrogen peroxide in the separation chamber. Finally, PTS chamber was connected and the catalysate was transferred from the separation chamber to the PTS chamber, leading to the color change of PTS, followed by using smartphone App to collect and analyze the image of PTS for bacterial determination. The simple biosensor enabled simple detection of Salmonella as few as 130 CFU/mL within 60 min and is promising for practical applications in the resource-limited regions due to its low cost, simple operation, and small size.


Assuntos
Técnicas Biossensoriais , Microfluídica , Microbiologia de Alimentos , Glucose , Salmonella typhimurium , Seringas
13.
Foods ; 11(7)2022 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-35407050

RESUMO

This study analyzed the changes of odor and taste in Daokou braised chicken during processing by GC-MS, GC-IMS, e-nose and e-tongue. The 75 and 55 volatile compounds identified in Daokou braised chicken by GC-MS and GC-IMS, respectively, included hydrocarbons, aldehydes, alcohols, terpenes, ketones, heterocyclics, esters, acids and phenols; among them, aldehydes, alcohols and ketones were the most abundant. The number and proportion of volatile compounds in Daokou braised chicken changed significantly (p < 0.05) in the process. The proportion of volatile compounds with animal fatty odor, such as aldehydes and alcohols, decreased, while that of esters, ketones and terpenes from spices with fruity fragrance increased, especially in the braising stage. An e-nose showed that the odor intensities of sulfur-containing and nitrogen oxide compounds were higher (p < 0.05) after the braising stage, but weakened after 2 h braising. An e-tongue showed that saltiness and richness increased significantly (p < 0.05) after braising. The results of these four techniques showed that braising promoted the release of flavor compounds, and was beneficial to salt penetration and umami release. However, long braising could lead to weakened flavor intensity and the introduction of bitterness and astringency. This study also found that GC-IMS and e-nose were more sensitive to trace compounds such as sulfur-containing and nitrogen oxide compounds, esters, acids and phenolics in Daokou braised chicken than GC-MS. The use of multiple technologies could provide more comprehensive flavor profiles for Daokou braised chicken during processing. This study provides insights into the control of flavor of Daokou braised chicken, and may be of practical relevance for the poultry industry.

14.
Spectrochim Acta A Mol Biomol Spectrosc ; 267(Pt 2): 120456, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34653807

RESUMO

The consumption of food infected with food-borne pathogens has become a global public health problem. Therefore, it is monitor food-borne infections to avoid health and financial consequences. The rapid detection and differentiation of bacteria for biomedical and food safety applications continues to be a significant challenge. Herein, we present a label-free surface-enhanced Raman scattering approach for separating harmful bacteria from food. The method relies on the ascorbic acid reduction method to synthesize silver nanoparticles (AgNPs) and a polydimethylsiloxane (PDMS) multi-hole filter membrane chip (AgNPs@PDMS multi-hole filter membrane chip). Surface-enhanced Raman spectroscopy (SERS) was used, followed by multivariate statistical analysis to differentiate five important food-borne pathogens, including Staphylococcus aureus, Salmonella typhimurium, Listeria monocytogenes, Clostridium difficiles and Clostridium perfringens. The results demonstrated that compared to normal Raman signals, the intensity of the SERS signal was greatly enhanced with an analytical enhancement factor of 5.2 × 103. The spectral ranges of 400-1800 cm-1 were analyzed using principal component analysis (PCA) and stepwise linear discriminant analysis (SWLDA) were used to determine the optimal parameters for the discrimination of food-borne pathogens. The first three principal components (PC1, PC2, and PC3) accounted for 87.3% of the total variance in the spectra. The established SWLDA model had 100% accuracy and cross-validation accuracy, which accurately distinguished the SERS spectra of the five species. In conclusion, the SERS technology based on the AgNPs@PDMS multi-hole filter membrane chip was useful for the rapid identification of food-borne pathogens and can be employed for food quality management.


Assuntos
Nanopartículas Metálicas , Análise Espectral Raman , Dimetilpolisiloxanos , Análise Discriminante , Prata
15.
Food Chem ; 352: 129352, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-33691206

RESUMO

A ratiometric fluorescence sensor system is proposed for detecting bis(2-ethylhexyl) phthalate (DEHP) in pork, which is based on aptamer recognition with molybdenum disulfide quantum dots and cadmium telluride quantum dots (MoS2 QDs/CdTe-Apta). Two signals exist in the system, among which the response signal is transmitted by CdTe-Apta. The amide condensation between aptamers and CdTe QDs shortens the distance between CdTe QDs and DEHP, thus quenching the fluorescence of CdTe QDs, possibly through a photoinduced electron transfer mechanism. The MoS2 QDs deliver the self-calibration signal, and the fluorescence of MoS2 QDs remains almost constant when co-existing with DEHP. Linearity (R2 = 0.9536) was established for the DEHP concentration range 0.005-3.0 mg·L-1, with a limit of detection of 0.21 µg·L-1. The system was successfully applied in the determination of DEHP in pork. The system has potential for the quantitative determination of DEHP in practical applications.


Assuntos
Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/metabolismo , Técnicas Biossensoriais/métodos , Dietilexilftalato/análise , Carne de Porco/análise , Animais , Compostos de Cádmio/química , Dietilexilftalato/metabolismo , Dissulfetos/química , Molibdênio/química , Pontos Quânticos/química , Espectrometria de Fluorescência , Suínos , Telúrio/química
16.
Spectrochim Acta A Mol Biomol Spectrosc ; 232: 117997, 2020 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-32062401

RESUMO

Clostridium perfringens (C. perfringens) has the ability to form metabolically-dormant spores that can survive food preservation processes and cause food spoilage and foodborne safety risks upon germination outgrowth. This study was conducted to investigate the effects of different AGFK concentrations (0, 50, 100, 200 mM/mL) on the spore germination of C. perfringens in four matrices, including Tris-HCl, FTG, milk, and chicken soup. C. perfringens spore germinability was investigated using near infrared spectroscopy (NIRS) combined with chemometrics. The spore germination rate (S), the OD600%, and the Ca2+-DPA% were measured using traditional spore germination methods. The results of spore germination assays showed that the optimum germination rate was obtained using 100 mM/L concentrations of AGFK in the FTG medium, and the S, OD600% and Ca2+-DPA% were 98.6%, 59.3% and 95%, respectively. The best prediction models for the S, OD600% and Ca2+-DPA% were obtained using SNV as the preprocessing method for the original spectra, with the competitive adaptive weighted resampling method (CARS) as the characteristic variables related to the selected spore germination methods from NIRS data. The results of the S showed that the optimum model was built by CARS-PLSR (RMSEV = 0.745, Rc = 0.897, RMSEP = 0.769, Rp = 0.883). For the OD600%, interval partial least squares regression (CARS-siPLS) was performed to optimize the models. The calibration yielded acceptable results (RMSEV = 0.218, Rc = 0.879, RMSEP = 0.257, Rp = 0.845). For the Ca2+-DPA%, the optimum model with CARS-siPLS yielded acceptable results (RMSEV = 44.7, Rc = 0.883, RMSEP = 50.2, Rp = 0.872). This indicated that quantitative determinations of the germinability of C. perfringens spores using NIR technology is feasible. A new method based on NIR was provided for rapid, automatic, and non-destructive determination of the germinability of C. perfringens spores.


Assuntos
Clostridium perfringens/crescimento & desenvolvimento , Microbiologia de Alimentos , Esporos Bacterianos/crescimento & desenvolvimento , Animais , Asparagina/metabolismo , Galinhas/microbiologia , Infecções por Clostridium/microbiologia , Clostridium perfringens/química , Contagem de Colônia Microbiana , Manipulação de Alimentos , Conservação de Alimentos , Frutose/metabolismo , Glucose/metabolismo , Humanos , Produtos da Carne/microbiologia , Leite/microbiologia , Espectroscopia de Luz Próxima ao Infravermelho , Esporos Bacterianos/química
17.
J Food Sci ; 83(1): 46-52, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29194607

RESUMO

To evaluate the role of Maillard reactions in the generation of flavor compounds in Jinhua ham, the reactions of glucose and ethanal with histidine and lysine, respectively, were studied by simulating the ripening conditions of Jinhua ham. The volatile products produced were analyzed using solid phase microextraction-gas chromatography/mass spectrometry. The results showed that 8 volatile compounds were generated by the reaction of glucose and histidine and 10 volatile compounds were generated by the reaction of glucose and lysine. Reactions of ethanal with lysine and with histidine both generated 31 volatile compounds that contributed to the flavor of Jinhua ham. This indicates that histidine and lysine related to Maillard reactions possibly play important roles in the generation of the unique flavor compounds in Jinhua ham. This research demonstrates that free amino acids participate in the generation of volatile compounds from Jinhua ham via the Maillard reaction and provides a basic mechanism to explain flavor formation in Jinhua ham. PRACTICAL APPLICATION: Jinhua ham is a well-known traditional Chinese dry-cured meat product. However, the formation of the compounds comprising its special flavor is not well understood. Our results indicate that Maillard reactions occur in Jinhua ham under ripening conditions. This work illustrates the contribution of Maillard reactions to the flavor of Jinhua ham.


Assuntos
Histidina/química , Lisina/química , Reação de Maillard , Produtos da Carne/análise , Suínos , Compostos Orgânicos Voláteis/análise , Acetaldeído/química , Animais , China , Manipulação de Alimentos/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Glucose/química , Microextração em Fase Sólida/métodos , Paladar
18.
Cogn Emot ; 31(6): 1083-1096, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-27249308

RESUMO

Emotion is widely agreed to have two dimensions, valence and arousal. Few studies have explored the effect of emotion on conflict adaptation by considering both of these, which could have dissociate influence. The present study aimed to fill the gap as to whether emotional valence and arousal would exert dissociable influence on conflict adaptation. In the experiments, we included positive, neutral, and negative conditions, with comparable arousal between positive and negative conditions. Both positive and negative conditions have higher arousal than neutral ones. In Experiment 1, by using a two-colour-word Flanker task, we found that conflict adaptation was enhanced in both positive and negative contexts compared to a neutral context. Furthermore, this effect still existed when controlling stimulus-response repetitions in Experiment 2, which used a four-colour-word Flanker task. The findings suggest emotional arousal enhances conflict adaptation, regardless of emotional valence. Thus, future studies should consider emotional arousal when studying the effect of emotion on conflict adaptation. Moreover, the unique role of the emotional context in conflict-driven cognitive control is emphasised.


Assuntos
Nível de Alerta , Cognição , Conflito Psicológico , Ajustamento Emocional , Emoções , Adulto , Feminino , Humanos , Masculino , Tempo de Reação , Adulto Jovem
19.
J Food Sci ; 81(4): M906-12, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26954724

RESUMO

The objective of this study was to predict the total viable counts (TVC) and total volatile basic nitrogen (TVB-N) in pork using an electronic nose (E-nose), and to assess the freshness of chilled pork during storage using different packaging methods, including pallet packaging (PP), vacuum packaging (VP), and modified atmosphere packaging (MAP, 40% O2 /40% CO2 /20% N2 ). Principal component analysis (PCA) was used to analyze the E-nose signals, and the results showed that the relationships between the freshness of chilled pork and E-nose signals could be distinguished in the loadings plots, and the freshness of chilled pork could be distributed along 2 first principal components. Multiple linear regression (MLR) was used to correlate TVC and TVB-N to E-nose signals. High F and R2 values were obtained in the MLR output of TVB-N (F = 32.1, 21.6, and 24.2 for PP [R2 = 0.93], VP [R2 = 0.94], and MAP [R2 = 0.95], respectively) and TVC (F = 34.2, 46.4, and 7.8 for PP [R2 = 0.98], VP [R2 = 0.89], and MAP [R2 = 0.85], respectively). The results of this study suggest that it is possible to use the E-nose technology to predict TVB-N and TVC for assessing the freshness of chilled pork during storage.


Assuntos
Nariz Eletrônico , Microbiologia de Alimentos , Embalagem de Alimentos/métodos , Conservação de Alimentos/métodos , Nitrogênio/análise , Carne Vermelha/análise , Compostos Orgânicos Voláteis/análise , Animais , Atmosfera , Temperatura Baixa , Contagem de Colônia Microbiana , Armazenamento de Alimentos/métodos , Odorantes/análise , Análise de Componente Principal , Carne Vermelha/microbiologia , Refrigeração , Suínos , Vácuo
20.
Artigo em Inglês | IBECS | ID: ibc-119268

RESUMO

The Behavioral Activation for Depression Scale (BADS), a four-factor (Activation, Aviodance/Rumination, Work/School impairment, and Social Impairment), 25-item scale, was to assess response contingent positive reinforcement (RCPR) that underlies the depression. So far, all the studies that examined its psychometric properties supported its usefulness in assessing RCPR in western culture. The purpose of the current study was to further evaluate psychometric properties of BADS that measures RCPR in a non-western culture. Based on a sample of 440 college participants from China, the results revealed that the four-factor of 25-item structure had a good fit to the data except for two items loaded differently from what was hypothesized. The data seemed to support BADS as a valid measure of RCPR and the results were discussed in the context of cultural differences (AU)


No disponible


Assuntos
Humanos , Depressão/psicologia , Psicometria/instrumentação , Escalas de Graduação Psiquiátrica Breve , Diversidade Cultural
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